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timp1  (R&D Systems)


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    R&D Systems timp1
    Timp1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 164 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/timp1+elisa+kit/Human+TIMP-1+Quantikine+ELISA+Kit/us12553903-314-17-45
    Average 95 stars, based on 164 article reviews
    timp1 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: A metabolic dysfunction-associated steatotic liver acinus biomimetic induces pancreatic islet dysfunction in a coupled microphysiology system
    Article Snippet: Albumin assays were performed in a 1:100 efflux dilutions by enzyme linked immunosorbent assay (ELISA) using commercial antibodies (Bethyl Laboratories, cat. no. A80-129A and A80-129P) and an ELISA accessory kit (Bethyl Laboratories, E101) with a human albumin standard prepared in house (MilliporeSigma, cat. no. 126658). .. COL1A1 was measured using the Human pro-collagen 1A1 ELISA kit (R&D Systems, cat. no. DY6220-05) in a 1:50 efflux dilution, and TIMP1 was measured using the TIMP1 ELISA kit (R&D Systems, cat. no. DTM100) with a efflux dilution of 1:20. .. Urea was measured using the Stanbio BUN liquid reagent for diagnostic set (Stanbio Laboratory, cat. no. SB-0580-250).

    Article Title: A metabolic-dysfunction associated steatotic liver acinus biomimetic induces pancreatic islet dysfunction in a coupled microphysiology system
    Article Snippet: Albumin assays were performed in a 1:100 efflux dilutions by enzyme linked immunosorbent assay (ELISA) using commercial antibodies (Bethyl Laboratories, cat. no. A80-129A and A80-129P) and an ELISA accessory kit (Bethyl Laboratories, E101) with a human albumin standard prepared in house (MilliporeSigma, cat. no. 126658). .. COL1A1 was measured using the Human pro-collagen 1A1 ELISA kit (R&D Systems, cat. no. DY6220-05) in a 1:50 efflux dilution, and TIMP1 was measured using the TIMP1 ELISA kit (R&D Systems, cat. no. DTM100) with a efflux dilution of 1:20. .. Urea was measured using the Stanbio BUN liquid reagent for diagnostic set (Stanbio Laboratory, cat. no. SB-0580-250).



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    Myeloid-specific Nogo-B deficiency alleviates liver injury and fibrosis. ( A ) Breeding scheme used to generate mice with myeloid-specific Nogo-B deletion. ( B ) Identification of myeloid-specific Nogo-B-deficient mice. ( C ) Serum Nogo-B concentrations in the 3 liver fibrosis models, as determined by ELISA. ( D ) Measurement of ALT and AST in the serum of the mice. ( E ) Collagen I and α-SMA mRNA expression in the murine livers was evaluated through qRT-PCR. ( F–H ) Liver sections from the mice were subjected to H&E and Sirius Red staining and Masson staining and α-SMA IHC analysis, and the proportions of Sirius red-positive and Masson-positive and a-SMA-positive regions were quantified. ( I–K ) Hepatic protein levels of collagen I, MMP-9, α-SMA, and <t>TIMP-1</t> were assessed via Western blotting; GAPDH served as the loading control. The data are presented as the means ± SEMs; n = 6 per group; original magnification, 10×; scale bars, 200 μm; ∗∗ P < .01.
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    Image Search Results


    Myeloid-specific Nogo-B deficiency alleviates liver injury and fibrosis. ( A ) Breeding scheme used to generate mice with myeloid-specific Nogo-B deletion. ( B ) Identification of myeloid-specific Nogo-B-deficient mice. ( C ) Serum Nogo-B concentrations in the 3 liver fibrosis models, as determined by ELISA. ( D ) Measurement of ALT and AST in the serum of the mice. ( E ) Collagen I and α-SMA mRNA expression in the murine livers was evaluated through qRT-PCR. ( F–H ) Liver sections from the mice were subjected to H&E and Sirius Red staining and Masson staining and α-SMA IHC analysis, and the proportions of Sirius red-positive and Masson-positive and a-SMA-positive regions were quantified. ( I–K ) Hepatic protein levels of collagen I, MMP-9, α-SMA, and TIMP-1 were assessed via Western blotting; GAPDH served as the loading control. The data are presented as the means ± SEMs; n = 6 per group; original magnification, 10×; scale bars, 200 μm; ∗∗ P < .01.

    Journal: Cellular and Molecular Gastroenterology and Hepatology

    Article Title: Macrophage Nogo-B Drives Liver Fibrosis

    doi: 10.1016/j.jcmgh.2025.101622

    Figure Lengend Snippet: Myeloid-specific Nogo-B deficiency alleviates liver injury and fibrosis. ( A ) Breeding scheme used to generate mice with myeloid-specific Nogo-B deletion. ( B ) Identification of myeloid-specific Nogo-B-deficient mice. ( C ) Serum Nogo-B concentrations in the 3 liver fibrosis models, as determined by ELISA. ( D ) Measurement of ALT and AST in the serum of the mice. ( E ) Collagen I and α-SMA mRNA expression in the murine livers was evaluated through qRT-PCR. ( F–H ) Liver sections from the mice were subjected to H&E and Sirius Red staining and Masson staining and α-SMA IHC analysis, and the proportions of Sirius red-positive and Masson-positive and a-SMA-positive regions were quantified. ( I–K ) Hepatic protein levels of collagen I, MMP-9, α-SMA, and TIMP-1 were assessed via Western blotting; GAPDH served as the loading control. The data are presented as the means ± SEMs; n = 6 per group; original magnification, 10×; scale bars, 200 μm; ∗∗ P < .01.

    Article Snippet: The membranes were probed with the following primary antibodies: Nogo-B (rabbit mAb, Thermo Fisher), USP14, p-RIPK3, and p-MLKL (rabbit mAbs, Abcam); α-SMA, collagen I, TIMP1, MMP9, caspase-1, cleaved caspase-1, pro-IL-1β, cleaved IL-1β, GAPDH, RIPK3, MLKL, CD11b, and LY6G (rabbit mAbs, Cell Signaling Technology); RIPK3 (mouse mAb, Cell Signaling Technology); USP14, NLRP3, and ASC (mouse mAbs, Proteintech); and Trim28 and Prpf19 (rabbit mAbs, Proteintech).

    Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR, Staining, Western Blot, Control